Fig. 5
From: Hmga2 is necessary for Otx2-dependent exit of embryonic stem cells from the pluripotent ground state
Hmga2 is involved in the regulation of Otx2 target genes upon the exit of embryonic stem cells (ESCs) from the pluripotent ground state. a ChIP-qPCR analysis of the association of Hmga2 to gene targets of Otx2 in wildtype (wt) epiblast-like stem cells (EpiLCs). The negative control (neg ctrl) corresponds to the region 10.1 kb upstream of the Hmga2 transcriptional start site. qPCR data represent means of three independent experiments (n = 3) ± SEM. b ChIP-qPCR analysis of the association of Hmga2 to gene targets of Otx2 in wt induced pluripotent stem cells (iPSCs) induced to differentiate. qPCR data represent means of two independent experiments (n = 2) ± SD. c The relative level of the indicated mRNAs was measured by qPCR in EpiLCs derived from ESCs transfected with si Ctrl and si Hmga2. qPCR data represent mean of three independent experiments (n = 3) ± SEM. *P <0.05, **P <0.01. d qPCR analysis of the expression of the indicated genes in wt and Hmga2 KO iPSC clones differentiated into EpiLCs. qPCR data represent means of two independent experiments (n = 2) ± SD for each of the indicated wt or Hmga2 KO iPSC clones