Fig. 8

Hmga2 assists Otx2 in the activation of the Hmga2 gene. a The association of Hmga2 to the genomic region upstream of its own gene was measured by ChIP-qPCR in epiblast-like stem cells (EpiLCs). qPCR data represent mean of independent biological replicates (n = 5) ± SEM. b Otx2 binding to the genomic region upstream of Hmga2 gene (left panel) was measured by ChIP-qPCR in EpiLCs derived from embryonic stem cells transfected with the indicated siRNAs. Data are presented as means of independent experiments (n = 4) ± SEM. *P <0.05. The right panels shows the Hmga2 binding to its own enhancer in EpiLCs upon suppression of Hmga2. Data are presented as means of independent experiments (n = 3) ± SEM. * P <0.05. c Model illustrating the cooperation of Otx2 and Hmga2 in transcriptional control during the exit from the pluripotent ground state