Fig. 9

Notch1 intracellular domain (NICD) generation is not affected in Syt1 KD PC12 cells. Parental and Syt1 KD PC12 cells were transfected with Myc-tagged, constitutively active Notch1 with deleted extracellular domain (NΔECD), which is an immediate substrate of the γ-secretase. The cells were treated with DMSO or γ-secretase inhibitor — DAPT. Transfection with a plasmid encoding Notch1 intracellular domain (NICD) was used as a control. Western blot analysis shows no difference in the production of the Notch1 intracellular domain, as detected by anti-myc antibody, between parental and Syt1 KD PC12 cells. Data are presented as the NICD/NΔECD ratio; mean ± SEM, n = 4. Statistical significance was determined using unpaired student t-test. Syt1 synaptotagmin 1