Identification of the novel activity-driven interaction between synaptotagmin 1 and presenilin 1 links calcium, synapse, and amyloid beta

Table 1 Mass spectrometry screen identified Syt1 as a novel synaptic PS1-binding protein that shows calcium-dependent profile of the interaction

PS1 bait	Binding protein	Accession number	Gene symbol	Unique/total peptides		Sequence coverage		Protein function
				Ca²⁺(+)	Ca²⁺(-)	Ca²⁺(+)	Ca²⁺(-)	Ca²⁺(+)
NT	Synaptotagmin 1	NP033332	SYT1	4/4	None	11.6 %	None	Calcium sensor for synaptic vesicle fusion and neurotransmitter release
L6-7	Synaptotagmin 1	NP033332	SYT1	16/26	2/2	32.1 %	5.0 %
L6-7	Catenin delta 1	NP031641	CTNND1	17/20	25/35	20.9 %	30.8 %	Known presenilin 1 interactor in the brain

The number of peptides identified and the sequence coverage for Syt1 in Ca²⁺ (-) and Ca²⁺ (+) conditions is shown. A known PS1 interacting protein, Catenin1 delta, is shown as Ca²⁺-independent control

ISSN: 1741-7007