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Fig. 3 | BMC Biology

Fig. 3

From: The human Na+/H+ exchanger 1 is a membrane scaffold protein for extracellular signal-regulated kinase 2

Fig. 3

Native mass spectrum (MS) and molecular model of the hNHE1cdt:iaERK2 complex. a Non-denaturing mass spectrum of the hNHE1-ERK2 complex, acquired from 200 mM ammonium acetate. Deconvolution was performed using the UniDec software and the individual charge series fits are displayed below, corresponding to NHE1 (blue), ERK2 (green), NHE1:ERK2 complex (red), and minor contribution of ERK2 dimer (orange). b Proposed model for the hNHE1-ERK2 interactions. The hNHE1cdt interacts with ERK2 at three sites, exploiting a D-domain and two F-sites (shown in green and blue, respectively). At the D-domain recognition site, K679, L684, and V686 of the hNHE1cdt D3-domain, as well as D318, D321, Y128, and H125 of the iaERK2 D-domain recognition site are shown as sticks

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BMC Biology

ISSN: 1741-7007

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