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Table 1 Apparent rate constants, k app for hNHE1cdt phosphorylation by aERK2, and effect of D-domain and F-site mutations

From: The human Na+/H+ exchanger 1 is a membrane scaffold protein for extracellular signal-regulated kinase 2

P-site k app (h-1)
  WT D3-AXA D1D2-(AXA)2 F1-A F2-AA
S693 0.3489 ± 0.0101 0.3488 ± 0.0112 0.3193 ± 0.0109 0.3000 ± 0.009 0.4435 ± 0.0177
S723/S726a 0.0821 ± 0.0018 0.0592 ± 0.0010 0.0610 ± 0.0011 0.0741 ± 0.0015 0.1274 ± 0.0018
S771 0.0088 ± 0.0015 0.0070 ± 0.0011 0.0066 ± 0.0011 0.0047 ± 0.0008 0.0088 ± 0.0015
T779 0.1648 ± 0.0022 0.1413 ± 0.0017 0.1572 ± 0.0021 0.1473 ± 0.0024 0.1441 ± 0.0018
S785 0.0305 ± 0.0016 0.0325 ± 0.0016 0.0302 ± 0.0017 0.0088 ± 0.0019 0.0120 ± 0.0021
  1. aFitting of S723 and S726 individually would require fitting to biexponentials due to their extensive crosstalk, which in turn reduces fitting accuracy. Therefore, only decrease of the S723 peak intensity was fitted, which reports on both S723 and S726 phosphorylation due to their crosstalk