Nuf subcellular distribution is modified by aPKC phosphorylation. a-b Third instar imaginal wing disc (a) and a section is shown in (b). c-d Representation of an apical (1) and a transversal view (2) showing in d the position of apical-lateral markers. e aPKC clones in wing discs (marked by the absence of aPKC, green) accumulate Rab11 (e’ and red in e) and Nuf (e” and blue in e) apically. f-h Subcellular distribution of wild-type (f), non-phosphorylatable (g) and phosphomimetic (h) Nuf in wing discs. Nuf driven by hh-Gal4 accumulates apically. NufS155A (g, magenta) reaches the apico-lateral membrane, marked by aPKC (green, co-localization in white). NufS155D is excluded from the apico-lateral membrane (h). In (f-h), upper panels show apical views, medial panels show sagittal views and lower panels are close-ups of the above. Yellow asterisks mark the apical distribution of the respective Myc-Nuf versions. Scale bar 10 μm. i Quantification of myc (red) compared to DPATJ (blue) levels in epithelia of nuf
1 homozygous wing disc expressing Myc-NufWT, Myc-NufS155A or Myc-NufS155D. Picks of DPATJ mark cell membranes. j Apico-cortical accumulation of NufS155A (red) is lost in aPKC-depleted cells marked by the absence of GFP (green, delimited by the yellow dotted line). Par3 (green) was used as membrane marker. k Quantification of Myc (red) and GFP/Par3 (blue) at the boundary between two clone cells (1) or two wild-type cells (2). Nuf nuclear fallout, aPKC atypical PKC, GFP green fluorescent protein.