Fig. 4

Irga6 co-localizes with the endoplasmic reticulum in Irgm3 ^−/− cells. a Irgm1 ^−/−, Irgm3 ^−/−, and Irgm1/Irgm3 ^−/− mouse embryonic fibroblasts (MEFs) were induced with 200 U/mL IFN-γ and transfected with pEYFP-calreticulin for 24 hours. Cells were fixed and stained with anti-Irga6 antibody (10D7). Representative microscopic images of Irga6 and calreticulin co-localization are shown. Arrows point at the Irga6 structures magnified at the end of each panel in the following array: upper left: Irga6, upper right: calreticulin, lower left: merge, lower right: phase contrast. Scale bars represent 10 μM. b Quantification of 4a, showing percent of Irga6 structures co-localizing with calreticulin; 50 cells per sample were quantified and the means of three independent experiments ± standard deviation are shown