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Table 1 Cooperativity and hierarchy of IRG loading to lysosomesa

From: Loss of the interferon-γ-inducible regulatory immunity-related GTPase (IRG), Irgm1, causes activation of effector IRG proteins on lysosomes, damaging lysosomal function and predicting the dramatic susceptibility of Irgm1-deficient mice to infection

IRGs Experiment Total lysosomes Lys. without IRGs Lys. with 1st IRG only Lys. with 2nd IRG only Lys. with 1st and 2nd IRG P valueb Independent or cooperative
Irga6 + Irgb6 1 1090 713 305 4 68 <0.0001 Cooperative
2 1477 768 536 14 159 <0.0001
Irga6 + Irgd 1 1692 880 503 18 291 <0.0001 Cooperative
2 1232 702 299 8 223 <0.0001
Irga6 + Irgb10 1 899 437 257 123 82 0.7995 Independent
2 1337 570 459 184 124 0.5973
Irgb10 + Irgb6 1 1060 866 144 2 48 <0.0001 Cooperative
  1. aLysosomes carrying or not carrying IRG proteins were identified microscopically by co-staining with antibodies against LAMP1 and different pairs of IRG proteins
  2. b P values were calculated in a χ2 analysis with 3 d.f. of the four categories of lysosome, based on expectation of random assortment of the two IRG proteins