Fig. 1

A quantitative genome-wide screen for regulators of AMP gene expression. a Simplified overview of the RNAi screen protocol, adapted from [19]. b Screenshots from the RNAi screen web interface. Left panel: example of results for two clones (insert at top right: the query box) that target the gene gck-3. Contrary to clone sjj_Y59A8A.c that passed the first round of duplicate screening, and for which the results of the second (quadruplicate) round are also displayed, sjj_Y59A8A.b only provoked a 15 % reduction in normalized GFP expression in one of the two first-round tests and so was not retained for the second round. The results for each test are linked to the primary data, which is displayed in the right panel for a single experiment. Users have the option of plotting GFP fluorescence against any or all of three parameters; shown here is GFP versus dsRed expression (in arbitrary units). c The ranked averages of the two values for normalized GFP expression for each of the 21,355 RNAi clones tested (21,223 unique clones, 132 present in duplicate), on a log scale. d The averages, on a linear scale, of the two values for normalized GFP expression for the last 1,355 RNAi clones. The 295 clones that were retested in a second round are indicated in red. e The averages, on a linear scale, of the two values for normalized GFP expression for the first 3000 RNAi clones. The 966 and 360 clones that passed first and second round screening are indicated in red and green, respectively. The results for selected known signaling components are indicated in black