B.al/rapaav and the B.al/rapaav homolog express pro-apoptotic genes differentially in cell-death mutants. All genotypes include nIs343, and some also contain †: him-5(e1490), ‡: him-8, °: nIs349, §: lon-1(e1820) dpy-17, : lon-1(e185), ★: unc-32.
a B.al/rapaav expresses P
::4xNLS::gfp in all genetic backgrounds studied. Percentage of animals with P
::4xNLS::gfp expression in death-fated B.al/rapaav during the early fourth larval stage, before B.al/rapaav death occurs. b The average intensity of P
::4xNLS::GFP in the nucleus is higher in B.al/rapaav than in the B.al/rapaav homolog in early fourth larval stage animals defective for cell death genes. A.U., arbitrary units. egl-1 (c, e) and ced-3 (d, f) transcripts are detectable in the presumptive B.al/rapaav (white arrow) in the tails of early fourth larval stage wild-type (c, d) and engulfment-defective (e, f) males. The genotype in c was nIs343; him-8; d
nIs343; him-5(e1490); nIs349; e, f
ced-12 ced-1; nIs343; him-8. Scale bars: 10 μm. g
ced-3 and egl-1 are differentially expressed between B.alapaav and B.arapaav. Only nuclear transcripts were counted. For each animal, the cell with the larger number of transcripts was classified as “high expression B.al/rapaav” and the other cell was classified as “low expression B.al/rapaav homolog.” When both cells had the same number of transcripts, one was arbitrarily labeled high and one low. This panel includes data from Fig. 2e. Animals contained nIs343 and †: him-5(e1490), ‡: him-8, °:nIs349. *: P < 0.005