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Fig. 3 | BMC Biology

Fig. 3

From: Both the apoptotic suicide pathway and phagocytosis are required for a programmed cell death in Caenorhabditis elegans

Fig. 3 and the homolog express pro-apoptotic genes differentially in cell-death mutants. All genotypes include nIs343, and some also contain †: him-5(e1490), ‡: him-8, °: nIs349, §: lon-1(e1820) dpy-17, : lon-1(e185), : unc-32. a expresses P egl-1 ::4xNLS::gfp in all genetic backgrounds studied. Percentage of animals with P egl-1 ::4xNLS::gfp expression in death-fated during the early fourth larval stage, before death occurs. b The average intensity of P egl-1 ::4xNLS::GFP in the nucleus is higher in than in the homolog in early fourth larval stage animals defective for cell death genes. A.U., arbitrary units. egl-1 (c, e) and ced-3 (d, f) transcripts are detectable in the presumptive (white arrow) in the tails of early fourth larval stage wild-type (c, d) and engulfment-defective (e, f) males. The genotype in c was nIs343; him-8; d nIs343; him-5(e1490); nIs349; e, f ced-12 ced-1; nIs343; him-8. Scale bars: 10 μm. g ced-3 and egl-1 are differentially expressed between B.alapaav and B.arapaav. Only nuclear transcripts were counted. For each animal, the cell with the larger number of transcripts was classified as “high expression” and the other cell was classified as “low expression homolog.” When both cells had the same number of transcripts, one was arbitrarily labeled high and one low. This panel includes data from Fig. 2e. Animals contained nIs343 and †: him-5(e1490), ‡: him-8, °:nIs349. *: P < 0.005

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