Fig. 3

The nature of horizontal gene transfer (HGT) between Magnaporthales and Colletotrichum. a Maximum likelihood (ML) tree of a putative dimethylaniline monooxygenase. This phylogeny provides an example of a gene transfer from Magnaporthales to Colletotrichum. b ML tree of a NACHT and TPR domain-containing protein. This phylogeny provides an example of a gene transfer from Colletotrichum to Magnaporthales. c Random sampling analysis of HGT gene clustering in the M. incrustans genome. We randomly sampled 93 genes from the M. incrustans data 5000 times (see Methods) and the number of genomic segments derived from these replicates (represented by the histogram) ranged from 0 to 7. In over 99.9 % (4955) of the replicates, six or less genomic segments resulted. Therefore, the chance is less than 0.1 % to generate the eight genomic segments that were observed in the empirical data (the thick black arrow). Similarly, the range of the genes that were included in the genomic segments was 0–14 with over 99.9 % of the gene numbers being 12 or less. Therefore, the chance is less than 0.1 % to generate a total of 18 genes that are contained in genomic segments. These results suggest that the enrichment of physical linkage in our HGT data cannot be explained solely by chance. d The proportion of carbohydrate-activating enzymes, transporters, and peptidases among the HGT set (gray color) in comparison to those in complete-genome data (white color). The results of significance test are indicated for each comparison