Fig. 5
The Z-cad sensor identifies and enables isolation of mesenchymal cells present among a heterogeneous population. a Fluorescent confocal microscopy was performed on parental HMLER cells containing the Z-cad dual sensor. Scale bar = 20 μm. b FACS was performed on parental HMLER cells to isolate mesenchymal-like GFPhiRFPlow/neg cells, which comprise the EMT signature. All other cells are designated as Bulk–EMT. c Cells from each group in b were collected, cytospun, and stained for pan-CK and vimentin. d RNA from each group collected in b was isolated and qRT-PCR performed for the indicated genes. Bulk–EMT cell values were set to 1.0 for each gene. Data were analyzed using a paired Student’s t test (n = 7 biological replicates). e Cells isolated from each group in b were plated and grown for 48 hours. Scale bar = 20 μm