Fig. 2
From: Somato-dendritic decoupling as a novel mechanism for protracted cortical maturation
Selective reduction of sodium channel α subunit expression in TeA neurons. a Confocal micrographs of TeA, hippocampal, and Oc1 neurons after immunolabelling with antibodies against the α-sodium channel subunit (Pan NaCH, green). Neurons were co-stained with DiI (red) to delineate the plasma membranes and NeuN (not shown) to verify their identity as neurons. White vertical lines on the XY image indicate the orthogonal plane where the Z-sections were taken. Blue vertical lines indicate the Z-location of the XY plane shown. In each group, cultures were processed for immunocytochemistry and DiI labelling completely in parallel, and images were captured under identical conditions. Scale bar = 10 μm. ** p < 0.01. b Quantification of peri-plasmalemmal channel expression (see Methods). n = 28, n = 33 and n = 19 for TeA, hippocampal and primary visual respectively. F (2,77) = 12.41 p < 0.0001, ANOVA and Tukey’s post hoc test (TeA significantly different than Oc1 and Hipp (p < 0.001). Hipp and Oc1 p > 0.05). c Confocal micrographs of TeA, hippocampal and Oc1 neurons after immunolabelling with antibodies against the sodium bicarbonate co-transporter (NBC, green). d Quantification of peri-plasmalemmal NBC expression. n = 27, n = 18 and n = 10 for TeA, hippocampal and primary visual respectively. There is no significant difference; F (2,52) = 0.76, p = 0.47. Cultures were also processed for immunocytochemistry and DiI labelling completely in parallel and images were captured under identical conditions