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Table 1 Quantitation of virus buds and membrane lengths for cryosections of MDMs infected with budding-arrested HIV-1 mutants

From: The intracellular plasma membrane-connected compartment in the assembly of HIV-1 in human macrophages

Mutant

No. of cell profiles analyseda

Cell surface

IPMC

% of buds in IPMC

Enrichment of buds in IPMCb

No. of virus buds

μm of membrane

Buds/μm of membrane

No. of virus buds

μm of membrane

Buds/μm of membrane

PTAP

D415c

7 (5)

26

2620

0.0099

1137

1378

0.8248

97.8

83 ×

PTAPYP

D428

9 (5)

4

2041

0.0020

719

931

0.7723

99.4

394 ×

D429

12 (11)

89

5278

0.0169

1149

1814

0.6333

92.8

38 ×

D432

6 (5)

44

2486

0.0177

417

483

0.8633

90.5

49 ×

Overall total

34 (26)

163

12,424

0.0131

3422

4607

0.7428

95.5

57 ×

  1. Monocyte-derived macrophages (MDMs) from four different donors were infected with the HIV-1 R3A mutants PTAP or PTAPYP, and infected MDMs located by on-grid immunolabelling. Electron microscopy images were recorded and analysed by counting the number of virus buds seen either at the cell surface or within intracellular plasma membrane-connected compartment (IPMCs). Linear lengths of surface or IPMC membrane within the sections were measured by tracing (see Methods)
  2. a The number of cell profiles for which viral buds were counted and membrane lengths measured (the number of distinct cells analysed is given in brackets)
  3. b The ratio of the density of buds/μm of membrane in the IPMC compared to the cell surface is shown as fold enrichment
  4. c Donor ID