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Table 1 Quantitation of virus buds and membrane lengths for cryosections of MDMs infected with budding-arrested HIV-1 mutants

From: The intracellular plasma membrane-connected compartment in the assembly of HIV-1 in human macrophages

Mutant No. of cell profiles analyseda Cell surface IPMC % of buds in IPMC Enrichment of buds in IPMCb
No. of virus buds μm of membrane Buds/μm of membrane No. of virus buds μm of membrane Buds/μm of membrane
PTAP
D415c 7 (5) 26 2620 0.0099 1137 1378 0.8248 97.8 83 ×
PTAPYP
D428 9 (5) 4 2041 0.0020 719 931 0.7723 99.4 394 ×
D429 12 (11) 89 5278 0.0169 1149 1814 0.6333 92.8 38 ×
D432 6 (5) 44 2486 0.0177 417 483 0.8633 90.5 49 ×
Overall total 34 (26) 163 12,424 0.0131 3422 4607 0.7428 95.5 57 ×
  1. Monocyte-derived macrophages (MDMs) from four different donors were infected with the HIV-1 R3A mutants PTAP or PTAPYP, and infected MDMs located by on-grid immunolabelling. Electron microscopy images were recorded and analysed by counting the number of virus buds seen either at the cell surface or within intracellular plasma membrane-connected compartment (IPMCs). Linear lengths of surface or IPMC membrane within the sections were measured by tracing (see Methods)
  2. a The number of cell profiles for which viral buds were counted and membrane lengths measured (the number of distinct cells analysed is given in brackets)
  3. b The ratio of the density of buds/μm of membrane in the IPMC compared to the cell surface is shown as fold enrichment
  4. c Donor ID