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Fig. 3 | BMC Biology

Fig. 3

From: A de novo silencer causes elimination of MITF-M expression and profound hearing loss in pigs

Fig. 3

Expression analysis of Mitf transcriptional variants and isoforms. a Schematics of splicing structure in the porcine Mitf transcript variants (leading to MITF-A, MITF-H and MITF-M) detected in the cochlea. The specific fragments of transcript variants used for quantitative PCR in this study are indicated by blue lines. b Expression profiles of MITF-A, MITF-H and MITF-M during cochlear development were examined by reverse transcription-PCR. MITF-M expression was present at detectable levels in the MitfR/R cochlea, but not in the Mitfr/r cochlea. c Immunoblotting analysis of MITF isoforms in the cochlea and skin. MITF-M expression was detectable in the MitfR/R cochlea and skin, but not in samples from the Mitfr/r pigs. d Differential level of expression of the Mitf exons in MitfR/r and Mitfr/r stria vascularis (SV). The M-exon showed a 11.5-fold decrease in the Mitfr/r SV. The fold change of each exon is estimated by comparing the normalized read count of each exon between MitfR/r and Mitfr/r SV in the RNA-seq assay. Raw data for this is provided in Additional file 5; sheet 4, Data of Mitf exon fold change

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