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Fig. 5 | BMC Biology

Fig. 5

From: A de novo silencer causes elimination of MITF-M expression and profound hearing loss in pigs

Fig. 5

Phenotypes of the Mitf-m knock-out mice. a Schematic of the Mitf-m targeting technical process. The region of the Mitf gene containing exons M, 2, 3, and 4 are shown at the top. The targeting vector with a floxed-neomycin cassette in the M-promoter/M-exon region is shown in the middle. The resultant Mitf gene portion after targeting (Mitfmi-ΔM allele) is shown at the bottom. b Mitf+/+ had a black coat color, and Mitfmi-ΔM/mi-ΔM had a white coat color and black eyes. c The auditory brainstem response thresholds were 20–30 dB SPL for the Mitf+/+ mice, and 100–110 dB SPL for the Mitfmi-ΔM/mi-ΔM mice (from 4 to 32 kHz). The raw data is provided in Additional file 5: sheet 6 Data of ABR tests (mice). d Mitf-m was not expressed at detectable levels in the Mitfmi-ΔM/mi-ΔM cochlea (red arrow), but was expressed at detectable levels in the Mitf+/+ cochlea. There was no difference observed between the expression levels of Mitf-a and Mitf-h in Mitfmi-ΔM/mi-ΔM and Mitf+/+ mice. Error bars in c and d indicate the standard deviations. Raw data in Additional file 5: sheet 7 Data of mouse Mitf qPCR (Ct) and sheet 8 Data of mouse Mitf qPCR (FC). e In the Mitfmi-ΔM/mi-ΔM cochlea, most of the stereocilias of inner hair cells (arrows) and outer hair cells (arrowheads) were fused or missing (stars). f The stria vascularis of Mitfmi-ΔM/mi-ΔM cochlea are significantly thinner and shorter than that of Mitf+/+ cochlea

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