Fig. 2

Co-expression of Sox2 with neural stem cells and cell-surface markers in the hindbrain. Representative flat-mount or section views of 18HH chick hindbrains that were co-stained for Sox2 and various cellular markers (n = 10 for each marker). A Hindbrain co-stained for Sox2 and Transitin. Flat-mounted views of single (a,b) or merged (c,d) channels. Higher magnification view of boundary area marked in (c) is presented in (d). Transverse section of single (e,f) or merged (g) channels of a boundary area. B Hindbrain co-stained for Sox2 and Pax6. Flat-mounted views of single (a,b) or merged (c,d) channels. Higher magnification view of boundary area marked in (c) is presented in (d). Transverse section of single (e,f) or merged (g) channels of a boundary area. C Hindbrain co-stained for Sox2 and GFAP. Flat-mounted views of single (a,b) or merged (c,d) channels. Higher magnification view of boundary area marked in (c) is presented in (d). Confocal Z-stack images of a boundary area (e–h). D Hindbrain co-stained for Sox2 and CSPG. Flat-mounted views of single (a,b) or merged (c,d) channels. Higher magnification view of boundary area marked in (c) is presented in (d). E Hindbrain co-stained for Sox2 and Hnk1. Flat-mounted views of single (a,b) or merged (c,d) channels. Higher magnification view of area marked in (c) is presented in (d). VZ = ventricular zone, MZ = mantle zone. Scale bars = 100 μm