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Fig. 3 | BMC Biology

Fig. 3

From: Type IV collagen drives alveolar epithelial–endothelial association and the morphogenetic movements of septation

Fig. 3

Decreased epithelial progenitors and increased type II pneumocytes in Col4a1 +/Δex41. aj Epithelial proliferation and differentiation were evaluated by staining with Ki67, NKX2.1, SOX9, and pSPC. a, b Overall proliferation evaluated by Ki67 is increased in Col4a1 +/Δex41 lungs. ch Double immunohistochemistry for Ki67 and NKX2.1 shows slightly active epithelial proliferation in NKX2.1 cells (arrows) in normal (ce) or Col4a1 +/Δex41 lungs at E18.5 (fh). l The bar charts show the percentage NKX2.1, SOX9 progenitors and pSPC+ cells over the total distal area of the lung of Col4a1 and Col4a2 mutants and wild type mice. Col4a1 +/Δex41 mutants have a statistically significant decrease of SOX9 cells and an increase of pSPC type II pneumocytes, while NKX2.1+ cells are unchanged compared with normal lungs at E18.5. j Real-time PCR of Nkx2.1, Sox9 and pSpc. Only Sox9 mRNA expression is decreased in Col4a1 +/Δex41. Gapdh was used as a normalizer. k The number of pSPC+ cells at P30 is increased in mutant lungs. l, o NKX2.1, m, p SOX9 and n, q pSPC localization in normal lungs displays a scattered pattern around the saccular walls (arrows), while in Col4a1 +/Δex41 mutants NKX2.1 and pSPC are clustered together (arrows). Scale bars = 200 μm in a, b, 50 μm in ch, and 200 μm in lq

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