Fig. 1

Dose-dependent effects of the MEK inhibitor U0126 on embryonic gene expression. Control blastula stages at 24 hours post fertilization (a, g, m) and embryos treated with increasing concentrations of U0126 (b–f, h–l, n–r). In situ hybridization on blastula stages using Nvsprouty (a–f), Nvbrachyury (g–l), or NvfgfA1 (m–r) antisense probes. All images are lateral views with the presumptive endomesoderm (animal pole, future oral pole) to the top. The insets correspond to animal pole views. Numbers in lower left corner correspond to the number of animals with phenotype pictured / total number of animals assayed