Fig. 3

U0126 treatment results in a global decrease in neurogenic transcription factors. mRNA in situ expression of Nvath-like (A), NvsoxB(2) (B), and NvashA (C) in control embryos treated with in 1/3× artificial seawater (ASW) with 0.1 % dimethyl sulfoxide (DMSO). Expression of Nvath-like (A’), NvsoxB(2) (B’), and NvashA (C’) in animals treated with 15 μm U0126 in 1/3× ASW with 0.1 % DMSO. Embryos were classified and quantified as the percent having normal expression, weak expression, or no expression. Refer to key in figure for classification of phenotypes. The phenotypic class with the highest percentage of embryos is indicated. All embryo images are of early gastrula stage. The main figure panels are ectodermal focal planes of lateral views with the presumptive oral side to the left. The insets show deeper focal planes used to confirm embryonic stage