Fig. 4

NvashA is insufficient to rescue neuronal loss resulting from U0126 treatment. mRNA in situ expression of each gene is indicated in control embryos raised in 1/3× artificial seawater (ASW) with 0.1 % dimethyl sulfoxide (DMSO) (A–H), compared to embryos treated with 15 μm U0126 in 1/3× ASW with 0.1 % DMSO (A’–H’), and compared to embryos injected with NvashA:venus mRNA and treated with 15 μm U0126 in 1/3× ASW with 0.1 % DMSO (A”–H”). Genes are referred to by either name or protein ID number used in the Nematostella genome database v1.0 (http://genome.jgi.doe.gov/Nemve1/Nemve1.home.html). Embryos were classified and quantified as the percent having normal expression, weak expression, or no expression. The phenotypic class with the highest percentage of embryos is indicated. Treatment with U0126 strongly reduced all neural gene expression (compare A’–H’ with A–H). Misexpression of NvashA in U0126-treated animals was not sufficient to rescue neuronal loss induced by U0126 treatment (compare A”–H” to both A–H and A’–H’). All animals in panels A–G, A’–G’, and A–-G” are aboral ectodermal focal planes of 48 hours post fertilization embryos (late gastrula stage). Embryos in panels H, H’, and H” are a lateral view with oral side facing towards the left