Fig. 5

NvashA regulates multiple neuronal subtypes. Shown are three-dimensional projections of two juvenile polyps shown with the oral side towards the top of the image. a Double fluorescent in situ hybridization of NvLWamide-like (red) and Nvserum amyloid A-like (green) in a late gastrula stage embryo. b The transgenic line for NvLWamide::mcherry expression is shown. Neural soma and neurites are observed throughout the body column and tentacles. Ectodermal neurons with three projections are observed in the body column (white arrow), which are not found in the other transgenic line. c The transgenic line for Nvserum amyloid A-like::mcherry is shown. There are many fewer neurons compared to NvLWamide::mcherry, but characteristic neurons are present. The U-shaped neuron that has two orally projecting neurites (pink arrow) is specific to this transgenic line. Both lines have neurons that are located just over the mesenteries and send projections orally and aborally in neural tracts overlaying the mesenteries (yellow arrows). In all images, asterisks indicate relative position of mouth