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Fig. 8 | BMC Biology

Fig. 8

From: MAPK signaling is necessary for neurogenesis in Nematostella vectensis

Fig. 8

NvashA regulates a subset of U0126-dependent salt-and-pepper expressed genes. a Relative fold change calculated from quantitative polymerase chain reaction analysis of triplicate injections of the NvashA morpholino (MO) versus a control MO (dark gray bars), or NvashA:venus-injected versus control venus-injected animals (light gray bars). Broadly expressed NvfgfRa, Nvsfrp1/5, Nvsix3/6, and NvotxC regional patterning genes were included as controls. Two positive control genes, 12533 and 242283, are included. The remaining genes are the salt-and-pepper expressed genes identified by the U0126 microarray. The red box indicates a region between 1.5× and −1.5× fold change that corresponds to an insignificant change in expression. The reciprocal phenotypes observed in NvashA MO and NvashA:venus mRNA-injected animals for Nvvsx-like (bd) and Nvath-like (eg) were confirmed by mRNA in situ hybridization. Embryos were classified and quantified as the percent having normal expression, weak expression, or no expression. The phenotypic class with the highest percentage of embryos is indicated. All embryo images are at the early gastrula stage. All images are ectodermal focal planes of aboral views. Error bars represent standard error

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