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Fig. 1 | BMC Biology

Fig. 1

From: A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large

Fig. 1

Principles of the approach. a By crossing a transgenic C. elegans strain expressing the Avi-tagged gene of interest (green) with different BirA driver lines (blue), biotinylation of the tagged protein (red) is accomplished in distinct tissues. b Schematic drawing of the N- and C-terminal tags designed. Tags contain the sequences encoding GFP (with introns), two Tobacco etch virus (TEV) cleavage sites, and the Avi-tag sequence

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