Fig. 3
De novo magnetite formation kinetics assay. a TEM micrographs of non-magnetic wildtype (WT) cells induced for magnetite formation upon addition of 100 μM iron at time zero. Cells are arranged by progression after iron addition. Black arrows indicate magnetite crystals position. b Growth (OD565nm) and magnetic response (Cmag) for iron-starved non-magnetic WT and mamK D161A cells treated with iron at time zero. c TEM micrographs of magnetite formation progression in mamK D161A cells. Doubling time was determined for WT and mamK D161A strains as 4.4 and 4.9 h, respectively. Scale bars: 1 μm