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Fig. 5 | BMC Biology

Fig. 5

From: Segregation of prokaryotic magnetosomes organelles is driven by treadmilling of a dynamic actin-like MamK filament

Fig. 5

MamK filament dynamics analysis by FRAP. Photobleaching of mCherry-MamK was used to follow the recovery of the fluorescence corresponding to the MamK filament during 10 min. (a) mCherry-MamK and (b) mCherry-MamKD161A translational fusions expressed from a chromosomal insertion from the native P mamAB promoter. (c) mCherry-MamK and (d) mCherry-MamKD161A translational fusions expressed from a replicative plasmid from the P mamAB promoter. The left panels show representative cells for this assay, indicating the selected bleached areas (white dashed circles) and fluorescence recovery progression. The pre-bleaching image is a composite of the bright field and fluorescence channel to display subcellular localization. The right panels show the quantification of the MamK filament fluorescence recovery over the time from the corresponding strain. Time point zero was measured immediately after laser pulse. The half-time fluorescence recovery is presented as t½ in each plot. Scale bars: 1 μm

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