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Fig. 8 | BMC Biology

Fig. 8

From: Segregation of prokaryotic magnetosomes organelles is driven by treadmilling of a dynamic actin-like MamK filament

Fig. 8

Analysis of MamJ dynamics. Photobleaching of EGFP and mCherry was used to follow the recovery of the fluorescence corresponding to MamJ. a MamJ-EGFP and b MamJ-mCherry translational fusions expressed from a Tn5-based chromosomal insertion and a replicative vector, respectively. White dashed circles indicate bleached areas. White dashed line acts a reference point. Red dashed line indicates signal progression. The right panels show the quantification of the MamJ fluorescence recovery over the time from the corresponding strain. Zero time was measured immediately after laser pulse. The half-time fluorescence recovery is presented as t½ in each plot. c MamJ-Dendra2 photoconversion upon mamK co-expression in MSR wildtype (WT). Arrow indicates appearance of MamJ signal at the cell pole. A 67 % of analyzed cells (n = 27) showed a putative polar appearance of the photoconverted protein. Green channel displays the filament prior to photoconversion. Red channel shows photoconverted the protein after a 405 nm laser pulse application. d Photobleaching of MamJ-mCherry upon mamK D161A co-expression in MSR WT. The white dashed circle indicates the bleached area while the right panels show the quantification of the MamJ fluorescence recovery over time. The half-time fluorescence recovery is presented as t½ in each plot. Scale bars: 1 μm

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