Fig. 5

Risk analysis studies to evaluate the use of transgenic males for SIT. a To test the potential for outcrossing to a related species, the percentage of inseminated females is shown after mating C. macellaria or FL12-56 males with C. macellaria females. As a control, FL12-56 males were also mated with the parental J06 females. At least six replicate experiments were performed. b The penetrance of the female lethal construct was evaluated in different genetic backgrounds. The number of males (black bar) and females (red bar) that developed from crosses of FL11-18B males with females from the indicated wild type strains are shown. c Study of longevity of the sexing strains at standard and cooler temperatures. Longevity at 25 °C (dark curves) and 19 °C (light curves) of males from the J06 parental strain, the FL12-56 and FL11-15 transgenic lines. Two replicates of longevity tests with 50 males were combined and Kaplan–Meier survival curves were estimated for each combination of temperature and strain