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Table 1 Dissociation constants (K d) for interactions between Cbl variants and UbcH5B–Ub

From: Casitas B-lineage lymphoma linker helix mutations found in myeloproliferative neoplasms affect conformation

Immobilized GST-Cbl variant Analyte K d (μM)
WT UbcH5B–Ub 84 ± 2
Y371F UbcH5B–Ub 84 ± 2
Y371H UbcH5B–Ub 39 ± 1
Y371C UbcH5B–Ub 36 ± 1
Y371A UbcH5B–Ub 30 ± 1
Y371S UbcH5B–Ub 16 ± 0.7
Y371D UbcH5B–Ub 10 ± 0.5
Y371E UbcH5B–Ub 9 ± 0.4
pY371 Y368F UbcH5B–Ub 2.6 ± 0.6
Y368F UbcH5B–Ub 49 ± 2
M222E UbcH5B–Ub 14 ± 0.6
  1. Standard errors of the mean are indicated