Fig. 1

Impt-1 knockdown activates the integrated stress response (ISR). a Immunoblotting of phosphorylated (P-eIF2α) and total eIF2α (T-eIF2α) in pools of day 1 adult N2 and impt-1 ^+/– mutant worms. DTT is a PERK activator used as a positive control to induce eIF2α phosphorylation [67]. b Same as a, but samples are from N2 worms that were maintained in control vector (L4440) or impt-1 RNAi from eggs. These experiments were repeated twice. Mean ± SEM of quantification is shown below the representative results. c Representative images showing the GFP expression of day 0 adult Patf-5::GFP reporter strains treated with L4440 or impt-1 RNAi from L1 larval stage. Worms were exposed for 3 hours with 5 mM DTT or vehicle prior to imaging. Dashed lines delimitate the body of the worm. Head and tail of worms are indicated by arrows. Average of mean GFP intensity per worm is shown in right panel (n = 15; *P < 0.0001 to L4440 RNAi; ^# P < 0.0001 to vehicle treated worms, two-way ANOVA). Data are presented as mean ± SEM. The experiment is representative of two independent trials