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Fig. 2 | BMC Biology

Fig. 2

From: IMPACT is a GCN2 inhibitor that limits lifespan in Caenorhabditis elegans

Fig. 2

Components of the GCN-2 branch of the integrated stress response (ISR) pathway are required for impt-1 knockdown-induced longevity. a Lifespan assays of N2 and impt-1 +/– (ok3233) mutants. (b–f) Lifespan assays on control (L4440) or impt-1 RNAi from L1: (b) N2 from CGC, (c) gcn-1(nc40), (d) gcn-2(ok871), (e) N2 from the Blackwell lab [control of atf-5(tm4397)], and (f) atf-5(tm4397). Values of median lifespan and statistics are reported in Additional file 10: Table S1. Survival curves were compared using the log-rank test. All experiments were repeated at least twice. Data demonstrate a representative experiment (experiment I in a) or the composite of multiple experiments (II–IV in b and c, II and IV in d, or V–VI in e and f)

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