Fig. 2

Orco contains a well-conserved putative calmodulin binding site (CBS). a Orco snake plot with each amino acid color-coded according to its level of conservation across 78 Orco orthologs. The indicated intracellular CBS falls within one of the most well-conserved regions of Orco. b Sequences of the wild-type Orco CBS in Orco^WT, the N-terminal and C-terminal Orco CBS deletion mutant Orco^CBSΔ, the single tryptophan deletion mutant Orco^W341Δ, and the charge reversal mutant Orco^RH344EE. All the UAS-Orco transgenes we generated also have an N-terminal mCherry tag (not shown). CBS calmodulin binding site