Fig. 4

CBS mutant Orco cannot enter the ciliated outer dendrites. a Staining of UAS-mCherry::Orco ^WT (II); Orco-GAL4, Orco ¹ (III) antennae with the 21A6 antibody, a marker of the ciliary base, reveals normal Orco trafficking from the OSN soma all the way to the ciliated outer dendrites. b Orco trafficking in UAS-mCherry::Orco ^CBSΔ (II); Orco-GAL4, Orco ¹ (III) flies is blocked at the ciliary base. Some Orco is visible in the OSN soma and inner dendritic segments, but nothing passes the ciliary constriction marked by the 21A6 signal. In a and b, we visualized Orco^WT and Orco^CBSΔ proteins using their mCherry tags, but we used a higher gain on the confocal microscope for Orco^CBSΔ. All scale bars, 10 μm. OSN olfactory sensory neuron