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Fig. 3 | BMC Biology

Fig. 3

From: Emergence of wheat blast in Bangladesh was caused by a South American lineage of Magnaporthe oryzae

Fig. 3

Reinoculation of seedlings with fungal strains isolated from infected wheat seeds. Germinated conidia, growth of mycelia, infection, and sporulation of strains used to artificially inoculate wheat, barley, and goosegrass. a A germinated three-celled pyriform conidia (arrow) with hyphal growth on water agar medium. b, c Culture of isolate BTJP 3-1 on PDA plate; upper (left) and reverse side (right). d Photograph showing a diamond-shaped, water-soaked lesion (initial stage of infection symptom, upper arrow) on a green wheat seedling leaf five days after conidial inoculation. e, f Development of an eye-shaped lesion with a gray center (arrows in e and f) on wheat leaves. g, h A gradual progression of symptoms (arrows) on wheat leaves. i–l Light micrographs showing massive conidia production (red arrow) on aerial conidiophores (black arrow) on artificially infected leaves of wheat cultivars Prodip (i) and Shatabdi (j), goosegrass (k), and barley (l). Photographs were taken by a camera attached to a microscope at 100× magnification. Scale bars in j, k, and l indicate 50 μm

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