Generation of an integrated Hieracium genomic and transcriptomic resource enables exploration of small RNA pathways during apomixis initiation

Table 1 Summary of genotypes and phenotypes of Hieracium plants used in the analysis and nucleic acids examined

Species (genotype)	Genetic constitution^a	Phenotype^b	MMC stage ovaries	FM stage ovaries	Leaves
H. praealtum (R35)	2n = 4x-1 = 35	Apomict LOA/LOP	mRNA sRNA	mRNA sRNA	sRNA
H. piloselloides (D36)	2n = 4x = 36	Apomict LOA/LOP	mRNA sRNA	mRNA sRNA	nd
H. piloselloides (D18)	2n = 2x = 18^c	Apomict LOA/LOP	nd	nd	DNA
H. praealtum (m115)	2n = 4x-1 = 35^d,e	Sexual loa/lop	mRNA sRNA	mRNA sRNA	sRNA
H. praealtum (m134)	2n = 4x = 36^e	Sexual^f loa/LOP	mRNA sRNA	mRNA sRNA	sRNA
H. pilosella (P36)	2n = 4x = 36	Sexual	mRNA sRNA	mRNA sRNA	nd

nd not done
^aIndicates ploidy of the sporophyte, and the chromosome number
^b LOA and LOP indicate apospory and autonomous seed formation are evident, respectively; loa and lop indicate their phenotypic absence
^cD18, a dihaploid derived from D36 (see introduction)
^dThe chromosome number of m115 is presumed to be the same as R35
^e m115 and m134 are derived from gamma-ray mutagenesis of R35
^fGametophytes in m134 ovules are sexually derived, but are still capable of autonomous seed development

ISSN: 1741-7007