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Fig. 4 | BMC Biology

Fig. 4

From: Oligomeric interface modulation causes misregulation of purine 5´-nucleotidase in relapsed leukemia

Fig. 4

Structural changes in the cN-II mutants captured by H/D exchange. a Perturbations of all analyzed mutants are observed mainly at the oligomeric interface. Regions with faster (red) or slower (blue) deuteration kinetics in the mutants (related to the wild-type protein) are highlighted on the cN-II tetramer. The perturbed regions are indicated only at two subunits (surface representation) of tetramer. Graphs (percentage of deuteration) show detailed kinetics of these regions (black circle wild type, blue triangle R367Q, red square R238W, green diamond L375F). b HDX provides additional insight into functionally important sites in cN-II proteins. In particular, faster exchange of active site region in the mutants and high level of deuteration for helix A in all cN-II variants are shown. In addition, HDX kinetics of structured regions that do not exhibit significant changes are shown for illustration

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