From: Oligomeric interface modulation causes misregulation of purine 5´-nucleotidase in relapsed leukemia
Enzyme | ATP | V max [μmol.min-1mg-1] | Km [mM] | n | kcat (s-1) | kcat/Km [mM.s-1] |
---|---|---|---|---|---|---|
Wild-type | – | 26.4 ± 4.7 | 33.5 ± 9.1 | 1.27 ± 0.38 | 29.5 ± 2.1 | 0.88 ± 0.25 |
Wild-type | + | 31.6 ± 1.8 | 2.9 ± 0.8 | 0.90 ± 0.14 | 35.3 ± 2.1 | 12.3 ± 3.3 |
R367Q | – | 31.4 ± 2.9 | 5.8 ± 1.7 | 0.88 ± 0.16 | 35.1 ± 3.3 | 6.0 ± 1.8 |
R367Q | + | 28.8 ± 1.3 | 2.8 ± 0.8 | 0.85 ± 0.10 | 32.2 ± 1.5 | 11.4 ± 2.1 |
R238W | – | 23.4 ± 1.9 | 12.1 ± 3.1 | 0.85 ± 0.09 | 26.1 ± 2.1 | 2.1 ± 0.6 |
R238W | + | 31.2 ± 2.5 | 0.52 ± 0.25 | 0.54 ± 0.15 | 34.8 ± 2.8 | 67 ± 33 |
L375F | – | 36.2 ± 3.9 | 12.2 ± 3.9 | 0.78 ± 0.10 | 40.5 ± 3.4 | 3.3 ± 1.1 |
L375F | + | 29.5 ± 2.3 | 0.16 ± 0.08 | 0.49 ± 0.16 | 33.0 ± 2.6 | 206 ± 105 |