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Table 1 Kinetic parameters of cN-II enzymes in the presence and absence of ATP

From: Oligomeric interface modulation causes misregulation of purine 5´-nucleotidase in relapsed leukemia

Enzyme

ATP

V max [μmol.min-1mg-1]

Km [mM]

n

kcat (s-1)

kcat/Km [mM.s-1]

Wild-type

26.4 ± 4.7

33.5 ± 9.1

1.27 ± 0.38

29.5 ± 2.1

0.88 ± 0.25

Wild-type

+

31.6 ± 1.8

2.9 ± 0.8

0.90 ± 0.14

35.3 ± 2.1

12.3 ± 3.3

R367Q

31.4 ± 2.9

5.8 ± 1.7

0.88 ± 0.16

35.1 ± 3.3

6.0 ± 1.8

R367Q

+

28.8 ± 1.3

2.8 ± 0.8

0.85 ± 0.10

32.2 ± 1.5

11.4 ± 2.1

R238W

23.4 ± 1.9

12.1 ± 3.1

0.85 ± 0.09

26.1 ± 2.1

2.1 ± 0.6

R238W

+

31.2 ± 2.5

0.52 ± 0.25

0.54 ± 0.15

34.8 ± 2.8

67 ± 33

L375F

36.2 ± 3.9

12.2 ± 3.9

0.78 ± 0.10

40.5 ± 3.4

3.3 ± 1.1

L375F

+

29.5 ± 2.3

0.16 ± 0.08

0.49 ± 0.16

33.0 ± 2.6

206 ± 105

  1. The n value refers to the calculated Hill coefficient