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Table 1 Kinetic parameters of cN-II enzymes in the presence and absence of ATP

From: Oligomeric interface modulation causes misregulation of purine 5´-nucleotidase in relapsed leukemia

Enzyme ATP V max [μmol.min-1mg-1] Km [mM] n kcat (s-1) kcat/Km [mM.s-1]
Wild-type 26.4 ± 4.7 33.5 ± 9.1 1.27 ± 0.38 29.5 ± 2.1 0.88 ± 0.25
Wild-type + 31.6 ± 1.8 2.9 ± 0.8 0.90 ± 0.14 35.3 ± 2.1 12.3 ± 3.3
R367Q 31.4 ± 2.9 5.8 ± 1.7 0.88 ± 0.16 35.1 ± 3.3 6.0 ± 1.8
R367Q + 28.8 ± 1.3 2.8 ± 0.8 0.85 ± 0.10 32.2 ± 1.5 11.4 ± 2.1
R238W 23.4 ± 1.9 12.1 ± 3.1 0.85 ± 0.09 26.1 ± 2.1 2.1 ± 0.6
R238W + 31.2 ± 2.5 0.52 ± 0.25 0.54 ± 0.15 34.8 ± 2.8 67 ± 33
L375F 36.2 ± 3.9 12.2 ± 3.9 0.78 ± 0.10 40.5 ± 3.4 3.3 ± 1.1
L375F + 29.5 ± 2.3 0.16 ± 0.08 0.49 ± 0.16 33.0 ± 2.6 206 ± 105
  1. The n value refers to the calculated Hill coefficient
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