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Table 2 Differentially modified lysine residues revealed from mass spectrometric study of protein crosslinking in the presence and absence of ATP

From: Oligomeric interface modulation causes misregulation of purine 5´-nucleotidase in relapsed leukemia

    Relative abundance (wild type compared to R367Q)
    Protein:BS2G molar ratio Protein:BS3 molar ratio
Peptide Modified residues ATP 1:20 1:50 1:20 1:50
343–361 Lys 344; Lys 359 77-23 ± 20 88-12 ± 12 47-53 ± 24 65-35 ± 1
+ 88-12 ± 12 89-11 ± 11 62-38 ± 33 52-48 ± 43
345–362 Lys 359; Lys 361 37-63 ± 4 48-52 ± 13 20-80 ± 11 19-81 ± 5
+ 55-45 ± 5 43-57 ± 23 53-47 ± 5 44-56 ± 3
345–361 Lys 359 24-76 ± 11 34-66 ± 15 17-83 ± 12 14-86 ± 14
+ 24-76 ± 8 32-68 ± 18 30-70 ± 8 17-83 ± 8
343–359 Lys 344 80-20 ± 16 82-18 ± 10 94-6 ± 4 76-24 ± 13
+ 84-16 ± 16 85-15 ± 4 100-0 93-7 ± 7
  1. Relative abundance refers to quantitative comparison between products obtained from wild-type cN-II and the R367Q mutant. Each value represents the mean and standard deviation from three experiments
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