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Fig. 1 | BMC Biology

Fig. 1

From: Geminin prevents DNA damage in vagal neural crest cells to ensure normal enteric neurogenesis

Fig. 1

Ablation of Gem from early pre-ENCCs results in cell cycle deficits. Cryosections through the foregut area of control (ae) and Wnt1Cre|Gem (fj) E9.5 embryos immunostained for GFP (green), Ki67 (red) and pH3 (white), and processed for EdU labelling (blue). be is an enlarged view of the area boxed in a. gj is an enlarged view of the area boxed in f. White arrows in b, c, g and h indicate GFP+ NC cells positive or not for EdU. Red and white arrowheads in d, e, i and j indicate GFP+ NC cells with a G2-like and M-like pH3 staining, respectively. k, quantification of NC (GFP+) cells that are positive for Ki67, proliferating (Ki67+;GFP+) NC cells that are in S-phase (EdU+) or in the G2 or M-phase (pH3+) of the cell cycle. Multiple t-tests without correction, *P value < 0.05, **P value < 0.01, ***P value < 0.001. Scale bars: (a, f) 100 μm, (be and gj) 25 μm

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