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Fig. 4 | BMC Biology

Fig. 4

From: Geminin prevents DNA damage in vagal neural crest cells to ensure normal enteric neurogenesis

Fig. 4

Ablation of p53 leads to accumulation of DNA damage in Wnt1Cre|Gem embryos. E10.5 embryo cryosections at the level of the stomach (af) and E12.5 gut cryosections at the level of the midgut (hm) of control p53 –/– (ac and hj) and Wnt1Cre|Gem p53 –/ (df and km), immunostained for GFP (green), γH2AX (cyan) and processed for TUNEL (red). Insets correspond to the areas in the panels indicated with arrows. g, n, Quantification of NC (GFP+) cells that have DNA damage (γH2AX+), undergo apoptosis (TUNEL+) or both (γH2AX+;TUNEL+). Multiple t-tests without correction, **P value < 0.01. Scale bars: (af, hm) 100 μm, (insets) 10 μm

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