Fig. 6
From: Geminin prevents DNA damage in vagal neural crest cells to ensure normal enteric neurogenesis
Ablation of Gem from ENCCs leads to low level DNA damage and largely normal gut colonisation. Cryosections at the level of the stomach of control (a–c) and Sox10CreER(i10)|Gem (d–f) E11.5 embryos, immunostained for GFP (green), γH2AX (cyan) and processed for TUNEL (red). Insets correspond to the areas in the panels indicated with arrows. g, Quantification of NC (GFP+) cells that have DNA damage (γH2AX+), undergo apoptosis (TUNEL+) or both (γH2AX+;TUNEL+). Multiple t-tests without correction, *P value < 0.05. Whole-mount gut preparations of control (h) and Sox10CreER(i10)|Gem (i) E12.5 embryos immunostained for GFP to visualise the distribution of ENCCs along the gut. Red arrows indicate the position of the most caudally located ENCCs in the gut preparations. Scale bars: (a–f) 100 μm, (h–i) 400 μm, (insets) 10 μm