Fig. 4

Expression of anti-angiogenic factor Ang2 by cranial neural crest cells. a HCR in situ image of HH St15 embryo with FoxD3 (green) and Ang2 (magenta). Scale bar 100 μm. b Ang2 probe only. Ang2 RNA can be seen in many locations including the eye, neural tube, cranial neural crest streams, and along the outer edge of the somites. c r4 neural crest stream in (a) with polyline drawn in yellow for intensity measurement. d Intensity profile along yellow line in (c) showing the overlap of the Ang2 and FoxD3 signals. Transverse section of HH St15 embryo at the r4 neural crest stream showing FoxD3 and Ang2 signals (e) and Ang2 only (f). Scale bar 40 μm. g RT-PCR of sorted r4 neural crest for beta-actin and Ang2 probes