Fig. 7
From: Angiopoietin 2 signaling plays a critical role in neural crest cell migration
Gain and loss of Ang2 in neural crest cultures affects the amount of phosphorylated FAK. Neural tubes electroporated with either a GFP control vector (a), Ang2-FL (b), or Ang2-shRNA (c) were plated on fibronectin/poly-l-lysine coated glass and allowed to grow for 24 h. Electroporated vector in blue. The cultures were then stained with HNK1 (red) and pFAK (green) antibodies. Scale bars 50 μm. Inset images in a, b, and c: individual filopodia in the pFAK channel showing the size measurement. Scale bar 3 μm. d The average pFAK cluster size. Each cluster of pFAK was individually measured by the spot detection algorithm (Imaris) based on the fluorescence in the pFAK channel, so thousands of clusters were used to calculate the average, SEM, and comparison between conditions. Control 4 neural tubes and n = 4179 pFAK clusters, Ang2-FL 4 neural tubes and n = 3182 pFAK clusters, 4 neural tubes and Ang2-shRNA n = 3298 pFAK clusters. All conditions are statistically different from each other with control vs. Ang2-FL p = 1 × 10–22, control vs. Ang2-shRNA p = 5 × 10–85, and Ang2-FL vs. Ang2-shRNA p = 1 × 10–132