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Fig. 1 | BMC Biology

Fig. 1

From: Functional characterisation of long intergenic non-coding RNAs through genetic interaction profiling in Saccharomyces cerevisiae

Fig. 1

TLC1 validates the SGA approach for interrogating the function of lincRNAs. a Schematic illustration of the SGA screening procedure used for studying lincRNAs. A query strain (MATα) carrying deletion of a lincRNA (white box) is mated to an array of 4309 single knockout strains (MATa), each lacking a non-essential protein-coding gene (white X mark). Following several selection steps, double mutant strains are isolated and their growth fitness is compared to the corresponding single deletion strains derived from a parallel control SGA screen. b Genetic interactions (GIs) of TLC1 associated with the three indicated Gene Ontology (GO) biological processes. Gene nodes are coloured according to their assigned GO annotation. Negative (bold letters) and positive (regular font) GIs that belong to the three GO terms are shown. Previously established TLC1 cofactors are highlighted in light grey boxes. c Venn diagram showing the significant overlap (P = 2 × 10–29) between genes classified as having a negative GI with TLC1 and EST1 in this study. The P value was generated using the hypergeometric test (phyper)

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