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Fig. 2 | BMC Biology

Fig. 2

From: Establishment of high reciprocal connectivity between clonal cortical neurons is regulated by the Dnmt3b DNA methyltransferase and clustered protocadherins

Fig. 2

Morphological and electrophysiological properties of recorded layer 4 neurons. a Barrel structure visualized by cytochrome oxidase reaction (CO) in a chimeric mouse produced using green fluorescent protein (GFP)-positive wild-type induced pluripotent stem cells. Scale bar: 200 μm. b Spiny stellate cells (biocytin-stained, red), and traces of the soma and dendrites from a wild-type (WT) nonchimeric (left) and a GFP-positive wild-type chimeric mouse (right). Green: GFP-positive cells without biocytin injection. Dashed line: barrel. Scale bar: 50 μm. ce Total dendritic length (c), number of dendritic branches (d), and Sholl analysis (mean ± SEM) (e) of spiny stellate cells in P18–20 wild-type nonchimeric mice (gray, n = 7 cells sampled from 3 mice) and chimeric mice (GFP-positive, green, n = 9 cells from 2 mice). A bar indicates the mean (c, d). f Representative traces of action potentials evoked by depolarizing current injection (300 pA) in current clamp mode in neurons from wild-type nonchimeric mice (WT, upper) and in GFP-positive (WT P-cell, middle) and GFP-negative neurons (WT N-cell, lower) from chimeric mice. gi Box-and-whisker plots showing the value (median, 25th to 75th percentiles, minimum to maximum) of the resting membrane potential (RMP, g), the threshold to induce action potentials (firing threshold (h), and the input resistance (i) in each group at P18–20. The number of cells is indicated above each plot

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