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Fig. 6 | BMC Biology

Fig. 6

From: Establishment of high reciprocal connectivity between clonal cortical neurons is regulated by the Dnmt3b DNA methyltransferase and clustered protocadherins

Fig. 6

Effect of Dnmt3b deficiency on the expression of cPcdh isoforms. a Distribution of mRNA signals (green) for γA3 (left) and γA7 (right) in wild-type (WT) chimeric mice. Scale bar: 100 μm. b, c mRNA signals (green) for γA3 (upper) and γA7 (lower) in cPcdh-knockout (KO) chimeric mice (b) and γA3 expression at higher magnification (c). All cells and cPcdh-KO cells were visualized by Hoechst 33342 staining (blue) and a probe for the td-Tomato gene (red), respectively. Scale bar: 20 μm (b) and 10 μm (c). d Distribution of mRNA signals (green) for γA3 (upper) and γA7 (lower) in clonal Dnmt3b-KO cells visualized by immunostaining with an anti-green fluorescent protein (GFP) antibody (red) in Dnmt3b-KO chimeric mice. Scale bar: 20 μm. e Proportion of γA3-positive (left graph) and γA7-positive (right graph) neurons in induced pluripotent stem cell-derived Dnmt3b-KO cells (magenta) and wild-type cells (green) observed in slices prepared from Dnmt3b-KO chimeric mice and wild-type chimeric mice, respectively. The proportion of host-derived wild-type neurons that expressed γA3 or γA7 is shown in gray. The number of analyzed slices is shown above each box-and-whisker plot (median, 25th to 75th percentiles, minimum to maximum). *P < 0.05, **P < 0.01 (Mann–Whitney U test)

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