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Fig. 1. | BMC Biology

Fig. 1.

From: Quantitative imaging of mammalian transcriptional dynamics: from single cells to whole embryos

Fig. 1.

Optical techniques useful for imaging the mammalian cell nucleus in space and time. a Single-molecule tracking (SMT) using epi-illumination, in which fluorescently labeled molecules within the laser focus (purple oval) are excited and their movements followed over time; a few representative single-molecule trajectories are depicted. b Fluorescence correlation spectroscopy (FCS), which analyzes the fluctuations in fluorescence intensity as molecules move in and out of the laser focus to obtain quantitative information on their dynamics; a representative intensity fluctuation trace (inset) and the autocorrelation function curve calculated from the trace are shown. c Photobleaching-based imaging techniques, depicting a small region of the nucleus (green) that has been selectively photobleached (dark green box); a typical FRAP curve is shown here (red arrowhead denotes photobleaching)

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