Fig. 3

Motor coordination and sensorimotor gating deficits in 3-month old iHip14 ^Δ/Δ mice. Over three consecutive days, 3-month-old mice were trained on a fixed-speed rotarod and tested on an accelerating rotarod on the fourth day (a). The number of falls (left) and the latency to the first fall (right) were recorded. iHip14 ^Δ/Δ mice fell off the rotarod more (two-way ANOVA: genotype p = 0.0053, p < 0.0001; interaction p = 0.9071; N = 14–16) and sooner (two-way ANOVA: genotype p = 0.0009, training day p < 0.0001, interaction p = 0.4581; N = 14–16) both during training and during testing. Mice were allowed to freely explore and climb a wire mesh container. iHip14 ^Δ/Δ mice climbed fewer times (b; ANOVA: p = 0.001; N = 13–18) but did not rear less (c; ANOVA: p = 0.65; N = 13–18) compared to control mice. Pre-pulse inhibition (PPI) was measured as the percentage of decrease in the magnitude (velocity) of startle with a pre-pulse compared to the magnitude of startle without a pre-pulse. iHip14 ^Δ/Δ mice had a PPI deficit compared to controls (d; repeated measures ANOVA: genotype p < 0.0001, pre-pulse p < 0.0001; N = 15–18)