Fig. 6

Impaired synaptic transmission in iHip14 ^Δ/Δ MSNs.MSNs in the central dorsal striatum were whole-cell patch clamped in acute coronal slices from 3-month-old mice. A representative trace of current-clamp membrane potential responses to a series of current injections (from –100 pA to 200 pA in 50-pA increments) is shown in (a). iHip14 ^Δ/Δ MSNs had the same resting membrane potential (RMP) (b: ANOVA: p = 0.68; N = 21–25), fired at the same rheobase current (c; ANOVA: p = 0.30; N = 21–25), and had the same membrane capacitance (d; ANOVA: p = 0.094; N = 31–43). iHip14 ^Δ/Δ mice had decreased frequency (f left; ANOVA: p < 0.0001; N = 19–23) but increased amplitude (f right; ANOVA: p < 0.0001; N = 19–23) of spontaneous excitatory post-synaptic currents (sEPSC), representative traces are shown in (e). iHip14 ^Δ/Δ mice had increased paired pulse ratios (h; PPR; two-way ANOVA: genotype p = 0.0001; pulse interval p < 0.0001; interaction p = 0.0047; N = 8–10), representative traces are shown in (g)